The 30-year-old singer told the magazine, "I feel more beautiful than I've ever felt because I've given birth. I have never felt so connected, never felt like I had such a purpose on this Earth."
"The best thing about having a daughter is having a true legacy," she adds. "The word 'love' means something completely different now."
Beyonce and rapper mogul hubby Jay-Z gave birth to their baby girl, Blue Ivy Carter, in January.
The 16-time Grammy winner is joined on the list by fellow "Most Beautiful Woman" hall of famer Julia Roberts, who has appeared in the issue 13 times, and Nicole Kidman, who makes her 10th appearance.
The feature also notes the gorgeous guys, including David Beckham, Blake Shelton, Ashton Kutcher, and this year’s Sexiest Man Alive, Bradley Cooper.
New mom and music superstar Beyonce was named People's 2012 "World's Most Beautiful Woman" Wednesday in the magazine's special double issue celebrating its 23rd year.