She opened the MTV Video Music Awards in September, and Janet Jackson will also open the American Music Awards this month.
Dick Clark Productions has announced the superstar will perform a "special medley" at the Nov. 22 awards show.
"We're thrilled about having Janet Jackson open this year's show," said American Music Awards producer Larry Klein. "She is an incredibly dynamic performer and I think the audience will be excited to see what she has in store."
This is Jackson's fourth time performing on the AMAs. She will release her new album "Number Ones" on Nov. 17.
Jackson, 43, joins a line-up of performers including Whitney Houston, Rihanna, Green Day, Shakira, Lil Wayne, Mary J. Blige, Keith Urban, Lady Gaga, Eminem, Jay-Z, Alicia Keys, Jennifer Lopez, Black Eyed Peas, Kelly Clarkson, Carrie Underwood, Daughtry and Adam Lambert.
2009 AMA Nominees
Favorite Male Pop/Rock Artist
Eminem (pictured), Michael Jackson and T.I.
Favorite Female Pop/Rock Artist
Beyonce (pictured), Lady Gaga and Taylor Swift
Favorite Pop/Rock Band, Duo or Group
Black Eyed Peas (pictured), Kings of Leon and Nickelback
Favorite Pop/Rock Album
Lady Gaga (pictured) / Fame, Michael Jackson / Number Ones and Taylor Swift / Fearless
Favorite Male Country Artist
Jason Aldean (pictured), Darius Rucker and Keith Urban
Favorite Female Country Artist
Reba McEntire, Taylor Swift (pictured) and Carrie Underwood
Favorite Male Rap/Hip-Hip Artist
Eminem, Jay-Z (pictured) and T. I.
Favorite Rap/Hip-Hop Album
Eminem / Relapse, Jay-Z / Blueprint 3 and T.I. (pictured)/ Paper Trail
Favorite Male Soul/R&B Artist
Jamie Foxx (pictured), Michael Jackson and Maxwell
Favorite Female Soul/R&B Artist
Beyonce, Keyshia Cole and Keri Hilson (pictured)
Favorite Soul/R&B Band, Duo or Group
Black Eyed Peas, Day26 (pictured) and Mary Mary
Favorite Soul/R&B Album
Beyonce / I Am... Sasha Fierce, Black Eyed Peas / The E.N.D. and Michael Jackson (pictured) / Number Ones
Hannah Montana: The Movie, Hannah Montana 3 and Twilight Soundtrack
Favorite Alternative Rock Artist
Green Day (pictured), Kings of Leon and Shinedown
Favorite Adult Contemporary Artist
Daughtry, Jason Mraz (pictured) and Taylor Swift
Favorite Latin Artist
Aventura (pictured), Luis Fonsi and Wisin Y Yandel
Favorite Contemporary Inspirational Artist
Jeremy Camp (pictured), Brandon Heath and Mary Mary
Artist of the Year
Kings of Leon (alternative)(pictured), Taylor Swift (country), Lady Gaga (pop/rock), Eminem (rap/hip hop) and Michael Jackson (soul/R&B)