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Has the love affair ended?! Are Jennifer Aniston and John Mayer calling it quits?

This couple is over, according to E! Online. A source tells the site the actress and crooner may have ended their relationship. "He broke up with her when she got back from her European tour," the source spilled. Aniston was traveling to promote "Marley & Me" with co-star Owen Wilson.

Jen, 40, and John, 31, shared a romantic evening at the Academy Awards last month, and days earlier they were spotted puckering up during a Valentine's Day Bahamas getaway.

J and J's reps would not give E! a comment.

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